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Two nitrogenous bisphosphonates, BAY 099 (Mg-ethylene-1,1-bisphosphonate) and BAY 100 (Mg-2-hydroxy-ethylene-1,1-bisphosphonate) were studied in experimental chronic graft-versus-host disease (GVHD) in mice. In vivo assays included evaluation of the capacity of both drugs to suppress human mixed lymphocyte reactions (MLR) and in vivo graft-versus-host reaction (GVHR) in C57BL/6 and BALB/c mice, and to modulate the severity of bone marrow transplant-induced GVHD in C57BL/6 mice. In vivo GVHR was induced by transplantation of C57BL/6 bone marrow cells (2 x 10(6)) into lethally irradiated BALB/c mice. In vitro MLR and GVHR were induced by the addition of 5 x 10(6) C57BL/6 spleen cells and 3 x 10(6 be359ba680
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